The main hp 27xq research problem of the paper is aimed at determining the proper functioning of Lake Gardno within the period 2012-2014 considered as hydrological years in reference to the physiochemical properties of its waters, water balance, thermal regime and water overturn.Lake Gardno is a representative of non-run-off lake geo-eco-systems; i
Effectiveness of orofacial myofunctional therapy in orthodontic patients: A systematic review
OBJECTIVE: The aim of the present systematic review was to determine the existence of scientific evidence demonstrating the effectiveness of orofacial myofunctional therapy (OMT) as an adjuvant to orthodontic treatment in individuals with orofacial disorders.A further aim was to assess the methodological quality of the studies included in the revie
Multiplexed tat-Targeting CRISPR-Cas9 Protects T Cells from Acute HIV-1 Infection with Inhibition of Viral Escape
HIV-1 cure strategy by means of proviral knock-out using CRISPR-Cas9 has been hampered by the emergence of viral resistance against d2 fleshripper the targeting guide RNA (gRNA).Here, we proposed multiple, concentrated gRNA attacks against HIV-1 regulatory genes to block viral escape.The T cell line were transduced with single and multiple gRNAs ta